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Regulatory effects of SPDEF expression on apoptosis-related protein expression in triple-negative breast cancer cells. (A) Western blot detection of apoptosis-related protein expression in MAD-MB-468 and MAD-MB-468 cell lines. (B-D) Quantitative analysis bar graphs of pro-apoptotic protein expression: Bax (B), <t>cleaved</t> <t>caspase-3</t> (C), and cleaved caspase-9 (D). (E,F) Quantitative analysis bar graphs of anti-apoptotic protein expression: Bcl-2 (E) and Bcl-xL (F). *, P<0.05; **, P<0.01; ***, P<0.001. GAPDH, glyceraldehyde-3-phosphate dehydrogenase; NC, negative control; OE, overexpression; SPDEF, SAM pointed domain-containing ETS transcription factor.
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Regulatory effects of SPDEF expression on apoptosis-related protein expression in triple-negative breast cancer cells. (A) Western blot detection of apoptosis-related protein expression in MAD-MB-468 and MAD-MB-468 cell lines. (B-D) Quantitative analysis bar graphs of pro-apoptotic protein expression: Bax (B), <t>cleaved</t> <t>caspase-3</t> (C), and cleaved caspase-9 (D). (E,F) Quantitative analysis bar graphs of anti-apoptotic protein expression: Bcl-2 (E) and Bcl-xL (F). *, P<0.05; **, P<0.01; ***, P<0.001. GAPDH, glyceraldehyde-3-phosphate dehydrogenase; NC, negative control; OE, overexpression; SPDEF, SAM pointed domain-containing ETS transcription factor.
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Regulatory effects of SPDEF expression on apoptosis-related protein expression in triple-negative breast cancer cells. (A) Western blot detection of apoptosis-related protein expression in MAD-MB-468 and MAD-MB-468 cell lines. (B-D) Quantitative analysis bar graphs of pro-apoptotic protein expression: Bax (B), cleaved caspase-3 (C), and cleaved caspase-9 (D). (E,F) Quantitative analysis bar graphs of anti-apoptotic protein expression: Bcl-2 (E) and Bcl-xL (F). *, P<0.05; **, P<0.01; ***, P<0.001. GAPDH, glyceraldehyde-3-phosphate dehydrogenase; NC, negative control; OE, overexpression; SPDEF, SAM pointed domain-containing ETS transcription factor.

Journal: Translational Cancer Research

Article Title: SPDEF downregulation promotes tumor aggressiveness and poor prognosis in triple-negative breast cancer

doi: 10.21037/tcr-2026-1-0130

Figure Lengend Snippet: Regulatory effects of SPDEF expression on apoptosis-related protein expression in triple-negative breast cancer cells. (A) Western blot detection of apoptosis-related protein expression in MAD-MB-468 and MAD-MB-468 cell lines. (B-D) Quantitative analysis bar graphs of pro-apoptotic protein expression: Bax (B), cleaved caspase-3 (C), and cleaved caspase-9 (D). (E,F) Quantitative analysis bar graphs of anti-apoptotic protein expression: Bcl-2 (E) and Bcl-xL (F). *, P<0.05; **, P<0.01; ***, P<0.001. GAPDH, glyceraldehyde-3-phosphate dehydrogenase; NC, negative control; OE, overexpression; SPDEF, SAM pointed domain-containing ETS transcription factor.

Article Snippet: Antibodies against cleaved caspase-3 and GAPDH (catalog numbers: 68773-1-Ig and 60004-1-Ig) were purchased from Wuhan Sanying Biotechnology Co., Ltd. (Wuhan, China), while the cleaved caspase-9 antibody (catalog number: 20750T) was obtained from Cell Signaling Technology (Danvers, MA, USA).

Techniques: Expressing, Western Blot, Negative Control, Over Expression